Regulation of Gene Expression by Gary H. Perdew, Jack P. Vanden Heuvel, Jeffrey M. Peters

Regulation of Gene Expression



Download Regulation of Gene Expression




Regulation of Gene Expression Gary H. Perdew, Jack P. Vanden Heuvel, Jeffrey M. Peters
Language: English
Page: 316
Format: pdf
ISBN: 1588292657, 9781588292650
Publisher: Humana Press

Regulation of Gene Expression: Molecular Mechanisms presents a comprehensive overview of methods and approaches for characterizing mechanisms of gene regulation. The text is appropriate both as a graduate textbook and a standard laboratory reference and provides the essential groundwork for an advanced understanding of the various mechanisms that may result in altered activity of a specific cell protein. Each of three sections explores mechanisms of gene regulation and expression, and presents methods and protocols for achieving specific experimental goals. Part I focuses on approaches for studying control of mRNA expression and determining target genes for a given transcription copy. Part II outlines the methods for determining how proteins can regulate each other by mediating synthesis, degradation, protein-protein interactions, and posttranslational modification. Part III explores how gene targeting techniques in mice can provide insight into protein function. This volume provides a clear, concise overview of the protocols and techniques used to examine chemically or disease-mediated alterations in gene expression in mammalian systems.

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